Adenovirus Purification

Vivascience introduces Vivapure AdenoPack 100, a ready-to-use kit for adenoviruspurification, to the market. The new kit includes all reagents and devices necessary for clarification, purification and concentration of adenovirus from HEK293 cells. The rapid two-hour purification protocol is based on Vivascience's ion exchangemembranes incorporated in AdenoPack syringe filter units. The device is unique in its capability to efficiently capture and remove large virus particles. When compared to chromatography media, membrane adsorbers provide large 3000 nm pores allowing unrestricted access and recovery of adenovirus from the charged adsorber surface. In a fast and simple procedure, recoveries of up to 1–5 x 1012 purified virus particles per mL can be obtained for in vitro transfections.