esiWay Silencing Resource

At RZPD we are producing esiWay Silencing Resources for highly specific gene knockdown in mammalian cell lines with esiRNAs. Generation of esiRNAs is done by in vitro transcription of T7 promotor-tailed PCR products into long dsRNAs and subsequent degradation of the dsRNA into pools of 21-23 bp siRNA molecules using recombinant dicer enzymes. Transfection of esiRNA into the target cells allows for gene specific knock down by the process of RNA interference.
The best way for specific gene silencing was proven by using RZPDs esiWay Silencing Resources in combination with the Roche Applied Science X-tremeGENE siRNA Dicer Kit and X-tremeGENE Transfection Reagent. Very high gene silencing efficiency, low cytotoxicity and no off-target effects were observed.
RZPD provides direct and open access to esiWay Silencing Resources for genes of the human and mouse genome via GenomeCubeTM, RZPDs multifunctional online search tool for genIDs or gene-specific sequence data.