Convenient single 2X premix includes T4 DNA Ligase and optimized buffer system, lowering pipeting steps. The kit facilitates highly-efficient ligation of cohesive, blunt-end, and TA cloning. Depending on DNA ends, efficient ligation is reached in either 5 minutes at 25°C or 30 minutes at 16°C. Ligation solution enforces successful transformation and helps recovery of modified plasmids, without purification. Precious samples are applied in volume as low as 10 µl.
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