Capturing an image like this is impossible using conventional imaging technology. The depth of focus on a stereo microscope is very shallow, meaning that images taken through such an instrument will have large areas that are out of focus. The only solution to this problem is advanced software algorithms that analyse a series of Z-stack images, deciding which parts of each image are in focus and discarding the rest. Prior scientific has partnered with a number of leading image analysis companies to provide the necessary hardware essential for applications. In this particular image, a stereo microscope fitted with a precision motorised focus motor from Prior was used to capture a tightly defined range of Z-stack images that were analysed, processed and recombined.
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