03/22/2004 • Microscopy / Imaging

FCS and FLIM Extensions

FCS is a method to measure concentration and diffusion rates quantitatively down to the signal molecule level. Leica´s new FCS is a sensitive, cross-correlation detection and analysis system that couples with the external port of the Spectral Confocal Microscope. Leica Microsystems also introduces FLIM, a tool to measure ion concentrations, intracellular signal transduction, FRET membrane potential, and more using time-correlated single photon counting technology. The method is available as a D FLIM system using a pulsed 405nm diode laser or as a MP FLIM system
using multiphoton excitation laser as the excitation source
and timer reference.
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