Sensitive Chemiluminescent Detection of Hydrogen Peroxide

Lumigen HyPerBlu, a novel chemiluminescent substrate from Beckman Coulter enables direct detection of hydrogen peroxide. Combining a broad dynamic range and bright, sustained chemiluminescence, the ready-to-use reagent offers convenience for high-throughput screening laboratories.

Reaction of the substrate with hydrogen peroxide rapidly generates sustained high-intensity luminescence for maximum sensitivity in solution assays. Direct detection increases accuracy and simplifies data analysis. When coupled with oxidases, the reagent also allows reliable, one-step, indirect detection of oxidases or their substrates. The reagent is provided as a single solution and does not require mixing. It is stable for one year when stored in an amber bottle at 2–8°C.