All Fret applications are now integrated into the physiology module of the Axiovision 4.7 microscopy software from Carl Zeiss. This enables cell and development biologists to use all of the softwares functionalities such as the enhancement of fluorescence signals and the suppression of image noise. Operation is very easy as only a few clicks are required. The software is used to determine the energy transfer portion between two adjacent protein molecules through fluorescence energy transfer (Fret), measure the distance between adjacent protein molecules below the microscope resolution and obtain quantitative temporal and spatial information about the bonding and interaction between proteins, lipids, enzymes, DNA and RNA in vivo.