Detection and Characterization of Protein Aggregates

Wyatt Technology has published a new application note to demonstrate the capability of its Dynapro Plate Reader dynamic light scattering (DLS) instrument for achieving rapid detection and characterization of protein aggregates. The Plate Reader was able to perform DLS measurements on samples within the wells of a well plate, without removing the samples or physically introducing any substances into the wells. As a result, this innovative instrument was able to allow for programmed measurements of many solution conditions quickly and easily with minimum user intervention. Protein systems often exhibit aggregation as a result of changes in environmental conditions, including changes in formulation pH, ionic strength, concentration, series of freeze-thaw cycles and forced aging.