Ready-to-use Target Specific Silenced Cells

Cell-based assays offer more physiological relevance for screening purposes. Knock-down of a specific gene expression becomes more attractive to obtain a cellular model, in which a gene of interest is specifically silenced. The SilenciX technology combines both these advantages with the creation of ready-to-use silenced cell lines, for use in fundamental research, drug discovery, screening and ADME-Tox. Access to such cell lines helps the researcher to focus on experimental questions without the need to first construct a cellular model. SilenciX is suitable for various functional assays and brings stability and robustness to repetitive experiments.

The efficacy of the SilenciX technology has been demonstrated in HeLa cells for proteins involved in DNA damage and repair pathways (XPA, XPC, DNA-PKcs, XRCC4 ) and signal transduction (p53, BRCA1). The validation of the silencing is routinely done by qPCR, with more than 70% knock-down each time. A large number of target-specific SilenciX cell lines are already available with even more being produced in our lab such as mTOR, SIRT1, HDAC1... SilenciX ready-to-use, virus-free cellular model will speed up research work. Customisation for both human cell type and targets can be performed upon request. Further information can be found at www.tebu-bio.com/to/silenciX.

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