StarGate® - The new dimension of combinatorial cloning

Transfer any gene of interest systematically into E. coli, yeast, mammalian (etc.) background with high speed and convenience to screen for the optimal host/tag combination.

StarGate® - Combinatorial Cloning in one Tube

StarGate offers rapid and highly efficient subcloning of an arbitrary gene – initially cloned into a Donor Vector - to simultaneously fuse it with many different genetic surroundings via transfer into Acceptor Vectors to generate Destination Vectors. The latter enable the efficient expression of your protein with various features (e.g. different tags and different promoters) in different hosts (Figs.1, 2).

Key Advantages of StarGate® are

• Minimal modification of the gene of interest due to short combinatorial sites (4 bases only)
• Easy-to-handle subcloning procedure
• Systematic screening of different elements (e.g. tags/promoters) in a variety of hosts
• Inherent high level cloning efficiency due to a directed reaction (no equilibrium)
• Availability of a multitude of combinatorial sites for combinatorial cloning
  

The TAGnology

StarGate is a technology that allows the systematic combination of promoters (i.e. hosts), purification tag sequences or other genetic elements with any gene of interest (GOI) in a convenient cloning system. The core element of this new technology is the site-specific combinatorial enzyme StarCombinaseTM, that makes cloning versatile, fast, easy and safe.

Custom StarGate® Services

Tailor-made Acceptor Vectors, Cloning, Protein Expression and Purification
Using our expertise and access to innovative technologies, IBA/IBA Biologics is able to offer a comprehensive service from gene to protein. Contact our experts at service@iba-go.com for your specific quote.