First Commercial 4Pi System

4Pi microscopy uses a special phase- and wavefront-corrected double-objective imaging system linked to a confocal scanner to enable 4-7 fold increased axial resolution over confocal & two photon microscopy. Even in living specimens, axial sections of ~100 nm are obtained. The system maintains all advantages of fast scanning, Acousto-Optical Beam
Splitting (AOBS) and Spectral Detection of the Leica TCS SP2 AOBS for routine operation. The first marked leap in resolution in commercial 3D fluorescence microscopy opens up new dimensions for research in cell and developmental biology

The spatial structure of live yeast cells, labelled with EGFP, has been revealed using the Leica TCS 4PI and subsequent three-dimensional rendering. The
image shows an axial resolution of 95 nm. Studies at this level of resolution
so far were unattainable with a fluorescence microscope and open up new
dimensions for research in structural and analytical biology.

The Leica TCS 4PI is available as a special limited edition for partnering institutions.

• 110 nm axial resolution
• Fast scanning
• Spectral detection
• Acousto-optical beam splitting (AOBS)

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