Assay for Enzymatic Screening Programs and Cell-based Assays

Promega Corporation has launched its ROSGlo H₂O₂ Assay. A non-horseradish peroxidase (HRP)-dependent, plate-based bioluminescent assay for detecting reactive oxygen species (ROS), the assay is designed to specifically detect hydrogen peroxide (H₂O₂) in both enzyme and cell-based applications.

It measures the activity of enzymes that generate or eliminate H₂O₂, and possesses many benefits over other commercially available assays for small molecule screening, including low false hit rate, signal stability, and compatibility with liquid handling.

Importantly for screening applications, the assay does not rely on a reaction catalyzed by HRP, which is known to cause a high number of false hits. The assay can also be used to measure changes in the level of ROS by directly detecting H₂O₂ in cultured mammalian cells after two reagent additions, with no cell sample preparation required, reducing variability and the number of cells required.