Combination of Optical Microscopy and AFM

PicoQuant has combined its time-resolved confocal fluorescence microscope MicroTime 200 with Brukers BioScope Catalyst Atomic Force Microscope (AFM). The synchronized acquisition of these two systems enables simultaneous recordings of AFM and optical images of the same sample region and makes new investigation schemes in the field of live cell imaging feasible.

The synchronized data acquisition enables scientists to analyze e.g. the impact of protein changes on cell shape and structure. It also allows high-resolution imaging by merging of sub nanometer topography with optically encoded functionality as well as investigations of inter- and intramolecular distances using force spectroscopy.