Leica Microsystems has now developed a Leica DMI6000 B with Adaptive Focus Control (AFC) for researchers who demand consistent multidimensional imaging. The fully automated AFC is based on the reflection of a light beam at a suitable surface (e.g. petri dish) to keep the distance between the objective and the specimen constant.
Available for both widefield (including TIRF) and confocal applications, AFC dynamically regulates the focus position, whenever or wherever the experiment requires it. For more flexibility, AFC can also be operated in stand-alone mode and requires neither a PC connection nor additional external control devices.