ForceRobot® 300 New Standard in Single Molecule Force Spectroscopy

The JPK ForceRobot® 300 is a fully automated force spectroscope that allows the real-time study of molecular interactions on the nanoscale. The key to obtaining meaningful results in force spectroscopy is statistics, and this is where the new ForceRobot technology is revolutionary. On the one side, the ­automated setup and continuous adjustments provide huge improvements in the efficiency of data collection. On the other side, the integration with optical techniques allows targeted measurements where the molecules of interest are located. These factors, combined with highest data quality and stability, open the field of single molecule force spectroscopy to a new level of results.

Applications:

• Protein (un)folding and receptor-ligand interaction
• Elastic response of DNA or muscle proteins
• Single molecule mechanical properties, e.g. synthetic biopolymers, carbohydrates or spider silk protein
• Localization of binding of small molecules on proteins (e.g. inhibitors on membrane proteins)
• Quantification of kinetics, affinity and energy landscapes of biological interactions
• Colloidal probe and Nanoindentation experiments
• Analysis of adhesion forces of single macromolecules