Image Capture at Two Wavelengths

Nikons Eclipse Ti Series inverted microscopes can capture images at two different wavelengths simultaneously, using dual cameras. Accelerating image acquisition while maintaining full frame resolution, the system is ideal for FRET and the capture of rapid dynamic cellular events using calcium or other ion-targeted probes, ratio probes, dual emission ratiometric dyes etc. The two cameras are positioned on the instruments back and side ports. Perfect registration between the two cameras is assured on installation to ensure that no information is lost during imaging. No further realignment or specialised alignment software is required. Even when the intensity difference between wavelengths is large, high-quality images can be captured by adjusting camera sensitivity for each wavelength.