A1Si High-speed Spectral Acquisition

Nikon Instruments groundbreaking A1 series of confocal laser scanner systems has now been even further enhanced by the launch of the A1Si spectral detector option which provides fast 32-channel spectral imaging with speeds of up to 16 fps at 512 x 64 pixels and real time spectral unmixing. This cell-friendly system captures full spectral data in just one fast scan protecting cells from further light exposure.

Fast fluorescence unmixing during image acquisition is possible, with a 512 x 512 pixel, 32-channel image unmixed in less than one second. Coupled with high-speed spectral imaging, a clearly resolved image with no crosstalk between closely overlapping fluorophore emissions can be created in real time.

A new virtual filter function provides the flexibility to handle any new fluorescence probes. Desired spectral ranges can be selected and the total intensity of each range adjusted individually. Broader band spectral imaging is achieved on the A1Si, which requires only one pass of the laser to capture all the spectral data in the sample.

Dedicated NIS-Elements C software for image capture, processing and analysis for confocal microscope applications is provided as standard, as well as analysis software for FRAP and FRET. NIS-Elements C provides total integrated control over microscope and camera.

The new systems, together with Nikons Ti inverted microscope, set a new standard for advanced time-lapse studies of rapid cellular interactions to bring biological imaging to life.

Key features

• Enhanced spectral imaging spectral detector option for A1/A1R systems 32-channel imaging in just one scan speeds of up to 16 fps at 512 x 64 pixels
• Real-time spectral unmixing 512 x 512 pixel image unmixed in less than one second
• V-filtering function freely utilises 32 channels flexibility to handle any new probes
• Ultimate cell protection one fast scan reduces light exposure during multiprobe studies
• NIS-Elements C software total integration and control of microscope and camera
• Ti inverted microscope combined with the Ti, the A1 series is Nikons most powerful and versatile confocal live cell imaging system