Protein Enrichment Technology

Bio-Rad Laboratories introduced the Proteominer technology for protein enrichment to facilitate the detection of medium and low abundance proteins in complex biological samples in a reproducible and quantitative process. The technology concentrates low abundance proteins and decreases the concentration of high abundance proteins using a large, highly diverse library of hexapeptides bound to chromatographic supports. The diverse hexapeptide library enables a potential ligand for every protein in the biological sample and is not limited to a small subset of known proteins like other depletion technologies. In addition to the enrichment of low abundance proteins, the high abundance protein concentration is drastically reduced rather than completely eliminated – hence proteins associated with the high abundance proteins are not lost.