BD PuraMatrix Peptide Hydrogel is a synthetic matrix that is used to create defined three-dimensional microenvironments for cell culture experiments. This material consists of standard amino acids (1% w/v) and 99% water. Under physiological conditions, the peptide component self-assembles into a three-dimensional hydrogel that exhibits a nanometer scale fibrous structure. The resulting hydrogel has been shown to promote the differentiation of cell types such as hepatocyte progenitor cells, neural stem cells, hippocampal neurons, and endothelial cells. Other potential applications include stem cell proliferation, tumour cell migration and invasion or angiogenesis assays.