01/08/2008 • Microscopy / Imaging

Olympus FV1000: Taking cLSM to another level

Confocal laser scanning microscopy provided scientists with the tools to see a much greater level of detail in cells. The Olympus FV1000 has taken this to an even higher level, with the added functionality and flexibility of the multi-laser combiner and SIM scanner.

  • Multiple lasers: The multi-laser combiner enables the easy integration and more flexible use of an unmatched number of lasers, providing a wider range of available wavelengths for high-resolution, confocal live cell imaging. The system supports up to six diode and gas lasers, enabling the selection of a large number of wavelengths from near UV to far red. Presently, laser diodes provide 405, 440, 473, 559 and 635 nm and the gas lasers provide 457, 488, 514.5, 543 and 594 nm.
  • SIM scanner: The FV1000 incorporates 2 laser scanners in a single compact design for simultaneous confocal fluorescence observation and independent laser light stimulation. Synchronization of these two functions ensures that rapid cellular reactions that occur during or immediately following stimulation are not overlooked.
  • Regions of interest: Any region of interest can be specified for stimulation and scanning independently, with unrestricted control of variations in timing, duration and intensity. The circular Tornado scan provides highly efficient photobleaching and photoactivation in contrast to standard raster-scan patterns.
  • Application flexibility: The SIM scanner and multi-laser combiner make the FV1000 the most suitable confocal microscope for a variety of applications, including FRAP, FLIP, photoactivation, photoconversion, uncaging, laser ablation and many others.
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