Novel Hot Start Technology

The mix does not use Taq antibodies which eliminates potential mammalian-source DNA contamination. Also, since the polymerase is not chemically-inactivated, no extensive heat-activation step is necessary which reduces damage to precious DNA samples. HotStart-IT™ Products from USB use a novel hot start method called primer-sequestration (Fig 1). In order to resolve problems like unwanted amplification products and primer-dimers, USB has combined their Taq Polymerase with a recombinant protein which binds and sequesters primers at lower temperatures. This technique effectively blocks DNA synthesis from mis-priming events. Following the initial denaturation step, the protein will be inactivated. This novel hot start method enhances many complex PCR reactions by increasing both: specificity and yield.

ExoSAP-IT® PCR Clean-up

ExoSAP-IT reagent is designed for simple, quick PCR clean-up for downstream applications, such as DNA sequencing or Single Nucleotide Polymorphism (SNP) analysis. When PCR amplification is complete, any unconsumed dNTPs and primers remaining in the PCR mixture will interfere with many down-stream applications. ExoSAP-IT® removes these contaminants. Add it directly to the PCR mixture and incubated at 37°C for 15 minutes. After this treatment, ExoSAP-IT is simply inactivated by heating to 80°C for 15 minutes.

ExoSAP-IT means:

• One-tube/One Step PCR Clean-Up – Add ExoSAP-IT directly to PCR product
• Elininates Spin Columns – Decreases time and expense while increasing yield
• Concerves PCR Samples – 100% recovery of both: short and long PCR products
• Removes Contaminating Primers and dNTPs – no interference in downstream applications
• Scalable – Economical for high throughput purification
• Simple Processing – Robotic-friendly, replaces beads, filtrations and plates
  

Biochemicals and Convenience Reagents

USB biochemicals and Ultrapure brand products meet the highest industry standards for quality. They are available in a variety of grades such as ACS Reagent Grade, CP Grade (Chemically Pure), USP Grade (USBioAnalyzed) and Molecular Biology Grade. Further USB offers an extensive selection of Ultrapure biochemical reagents in premixed, ready-to-use formats for convenience in any lab.