Site-Directed Mutagenesis Kit

Finnzymes Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions or deletions in any type of plasmid DNA. Phusion Hot Start High-Fidelity DNA Polymerase in the kit ensures highly processive, exponential PCR amplification. The protocol comprises only three steps. Phosphorylated primers are designed to introduce the desired mutation(s) so that they anneal back to back to the plasmid. Phusion Hot Start DNA Polymerase amplifies the plasmid. The mutated plasmid is then circularized in a 5-minutes ligation and transformed into bacteria.